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antibody against the neural stem cell marker sox2 millipore ab5603  (Millipore)


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    Millipore antibody against the neural stem cell marker sox2 millipore ab5603
    Antibody Against The Neural Stem Cell Marker Sox2 Millipore Ab5603, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sox2+(ab5603)/sox2+antibody/pmc12204135-290-14-19
    Average 90 stars, based on 1 article reviews
    antibody against the neural stem cell marker sox2 millipore ab5603 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Morphologic and Immunohistochemical Features of Malignant Peripheral Nerve Sheath Tumors and Cellular Schwannomas
    Article Snippet: .. Ki-67 labeling index was estimated as percent positive nuclei in the area of highest labeling using online software (ImmunoRatio©, version 1.0c, Tampare, Finland), Ki-67 stain was not performed for malignant peripheral nerve sheath tumors from WUSTL. table ft1 table-wrap mode="anchored" t5 TABLE 1: caption a7 Marker Clone Source Dilution Antigen retrieval S100 S100 Rabbit polyclonal Ventana 1:2 CC1-120 min SOX10 SOX10 Rabbit polyclonal Cell Marque 1:50 CC1-60 min Neurofibromin NFC Mouse monoclonal DKFZ 1:4 CC2-56 min SOX2 AB5603 Rabbit polyclonal EMD Millipore 1:200 CC1-32 min p75NTR NGFR5 Mouse monoclonal Abcam 1:100 CC1-32 min p16 JC8 Mouse monoclonal Santa Cruz 1:50 CC1-32 min p53 DO-7 Mouse monoclonal Dako 1:50 CC1-32 min EGFR 3C6 Mouse monoclonal Ventana 1:2 CC1- 32 min MIB1 30-9 Rabbit monoclonal Ventana 1:1 CC1-32 min Open in a separate window CC1 : Cell conditioner 1 (pH 9), Ventana (Ventana Medical Systems, Tucson, AZ, USA); CC2 : Cell conditioner 2 (pH 6), Ventana (Ventana Medical Systems); min: Minutes. ..

    Software:

    Article Title: Morphologic and Immunohistochemical Features of Malignant Peripheral Nerve Sheath Tumors and Cellular Schwannomas
    Article Snippet: .. Ki-67 labeling index was estimated as percent positive nuclei in the area of highest labeling using online software (ImmunoRatio©, version 1.0c, Tampare, Finland), Ki-67 stain was not performed for malignant peripheral nerve sheath tumors from WUSTL. table ft1 table-wrap mode="anchored" t5 TABLE 1: caption a7 Marker Clone Source Dilution Antigen retrieval S100 S100 Rabbit polyclonal Ventana 1:2 CC1-120 min SOX10 SOX10 Rabbit polyclonal Cell Marque 1:50 CC1-60 min Neurofibromin NFC Mouse monoclonal DKFZ 1:4 CC2-56 min SOX2 AB5603 Rabbit polyclonal EMD Millipore 1:200 CC1-32 min p75NTR NGFR5 Mouse monoclonal Abcam 1:100 CC1-32 min p16 JC8 Mouse monoclonal Santa Cruz 1:50 CC1-32 min p53 DO-7 Mouse monoclonal Dako 1:50 CC1-32 min EGFR 3C6 Mouse monoclonal Ventana 1:2 CC1- 32 min MIB1 30-9 Rabbit monoclonal Ventana 1:1 CC1-32 min Open in a separate window CC1 : Cell conditioner 1 (pH 9), Ventana (Ventana Medical Systems, Tucson, AZ, USA); CC2 : Cell conditioner 2 (pH 6), Ventana (Ventana Medical Systems); min: Minutes. ..

    Staining:

    Article Title: Morphologic and Immunohistochemical Features of Malignant Peripheral Nerve Sheath Tumors and Cellular Schwannomas
    Article Snippet: .. Ki-67 labeling index was estimated as percent positive nuclei in the area of highest labeling using online software (ImmunoRatio©, version 1.0c, Tampare, Finland), Ki-67 stain was not performed for malignant peripheral nerve sheath tumors from WUSTL. table ft1 table-wrap mode="anchored" t5 TABLE 1: caption a7 Marker Clone Source Dilution Antigen retrieval S100 S100 Rabbit polyclonal Ventana 1:2 CC1-120 min SOX10 SOX10 Rabbit polyclonal Cell Marque 1:50 CC1-60 min Neurofibromin NFC Mouse monoclonal DKFZ 1:4 CC2-56 min SOX2 AB5603 Rabbit polyclonal EMD Millipore 1:200 CC1-32 min p75NTR NGFR5 Mouse monoclonal Abcam 1:100 CC1-32 min p16 JC8 Mouse monoclonal Santa Cruz 1:50 CC1-32 min p53 DO-7 Mouse monoclonal Dako 1:50 CC1-32 min EGFR 3C6 Mouse monoclonal Ventana 1:2 CC1- 32 min MIB1 30-9 Rabbit monoclonal Ventana 1:1 CC1-32 min Open in a separate window CC1 : Cell conditioner 1 (pH 9), Ventana (Ventana Medical Systems, Tucson, AZ, USA); CC2 : Cell conditioner 2 (pH 6), Ventana (Ventana Medical Systems); min: Minutes. ..

    Marker:

    Article Title: Morphologic and Immunohistochemical Features of Malignant Peripheral Nerve Sheath Tumors and Cellular Schwannomas
    Article Snippet: .. Ki-67 labeling index was estimated as percent positive nuclei in the area of highest labeling using online software (ImmunoRatio©, version 1.0c, Tampare, Finland), Ki-67 stain was not performed for malignant peripheral nerve sheath tumors from WUSTL. table ft1 table-wrap mode="anchored" t5 TABLE 1: caption a7 Marker Clone Source Dilution Antigen retrieval S100 S100 Rabbit polyclonal Ventana 1:2 CC1-120 min SOX10 SOX10 Rabbit polyclonal Cell Marque 1:50 CC1-60 min Neurofibromin NFC Mouse monoclonal DKFZ 1:4 CC2-56 min SOX2 AB5603 Rabbit polyclonal EMD Millipore 1:200 CC1-32 min p75NTR NGFR5 Mouse monoclonal Abcam 1:100 CC1-32 min p16 JC8 Mouse monoclonal Santa Cruz 1:50 CC1-32 min p53 DO-7 Mouse monoclonal Dako 1:50 CC1-32 min EGFR 3C6 Mouse monoclonal Ventana 1:2 CC1- 32 min MIB1 30-9 Rabbit monoclonal Ventana 1:1 CC1-32 min Open in a separate window CC1 : Cell conditioner 1 (pH 9), Ventana (Ventana Medical Systems, Tucson, AZ, USA); CC2 : Cell conditioner 2 (pH 6), Ventana (Ventana Medical Systems); min: Minutes. ..

    other:

    Article Title: PDGFRα + Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors
    Article Snippet: 1/1,000 WB Gata6 AF1700 R&D systems 1/1,000, 1/400 WB, IF Donkey anti-Ms IgG-HRP SC-2314 Santa Cruz B.

    Article Title: PDGFRα + Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors
    Article Snippet: 1/1,000 WB Gata6 AF1700 R&D systems 1/1,000, 1/400 WB, IF Donkey anti-Ms IgG-HRP SC-2314 Santa Cruz B.

    Article Title: Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro.
    Article Snippet: The antibodies used were as following: Oct4 (Santa Cruz sc-8628, 1/100); Sox2 (Millipore AB5603, 1/400); Nanog (Abcam ab80892, 1/400); Tfe3 (Sigma HPA023881, 1/300); 5mC (Eurogentec B1-MECY-1000, 1/200); 5hmC (Active Motif 39769, 1/500); H3K27me3 (Millipore 07-449, 1/200); Pax6 (covance PRB-278P, 1/500); Tuj1 (covance MMS435P, 1/1000); Vimentin (Santa Cruz sc-7557, 1/100); Nestin (Millipore MAB353, 1/200); eGFP (millipore MAB3580, 1/500); VASA (abcam ab13840, 1/200); Alexa-488- and Cy3-conjugated secondaryantibodies were obtained from Jackson Immuno-research.

    Incubation:

    Article Title: WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma
    Article Snippet: .. Protein Reference Source Technique Dilution Incubation WNT6 ab50030 Abcam IHC 1:450 O/N at 4 ̊C IF 1:1 000 O/N at 4 ̊C ab154144 WB 1:500 O/N at 4 ̊C Ki-67 550609 BD Biosciences IHC 1:200 O/N at 4 ̊C Cyclin D1 2978 Cell Signaling IHC 1:100 O/N at 4 ̊C SOX2 AB5603 EMD Millipore IHC 1:500 O/N at 4 ̊C IF 1:300 O/N at 4 ̊C BCL2 2870 Cell Signaling IHC 1:200 O/N at 4 ̊C NESTIN MAB5326 EMD Millipore IF 1:100 1h at RT rabbit IgG (H+L) – Alexa fluor® 594 A-11012 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark mouse IgG (H+L) – Alexa fluor® 488 A-11001 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark p-STAT3 Y705 9145 Cell Signaling WB 1:2 000 O/N at 4 ̊C STAT3 9132 Cell Signaling WB 1:1 000 O/N at 4 ̊C non-p-β-catenin S33/S37/T41 4270 Cell Signaling WB 1:5 000 O/N at 4 ̊C β-catenin 610153 BD Bioscience WB 1:2 000 O/N at 4 ̊C p-AKT S473 4051 Cell Signaling WB 1:1 000 O/N at 4 ̊C AKT 2920 Cell Signaling WB 1:2 000 O/N at 4 ̊C α-tubulin sc-23948 Santa Cruz Biotechnology WB 1:1 000 1h at RT mouse IgG – HRP conjugated sc-2031 Santa Cruz Biotechnology WB 1:1 000 1h at RT rabbit IgG – HRP conjugated sc-2004 Santa Cruz Biotechnology WB 1:1 000 1h at RT IHC: Immunohistochemistry; IF: Immunofluorescence; WB: Western Blot; O/N: Overnight; RT: Room Temperature ..

    Immunohistochemistry:

    Article Title: WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma
    Article Snippet: .. Protein Reference Source Technique Dilution Incubation WNT6 ab50030 Abcam IHC 1:450 O/N at 4 ̊C IF 1:1 000 O/N at 4 ̊C ab154144 WB 1:500 O/N at 4 ̊C Ki-67 550609 BD Biosciences IHC 1:200 O/N at 4 ̊C Cyclin D1 2978 Cell Signaling IHC 1:100 O/N at 4 ̊C SOX2 AB5603 EMD Millipore IHC 1:500 O/N at 4 ̊C IF 1:300 O/N at 4 ̊C BCL2 2870 Cell Signaling IHC 1:200 O/N at 4 ̊C NESTIN MAB5326 EMD Millipore IF 1:100 1h at RT rabbit IgG (H+L) – Alexa fluor® 594 A-11012 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark mouse IgG (H+L) – Alexa fluor® 488 A-11001 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark p-STAT3 Y705 9145 Cell Signaling WB 1:2 000 O/N at 4 ̊C STAT3 9132 Cell Signaling WB 1:1 000 O/N at 4 ̊C non-p-β-catenin S33/S37/T41 4270 Cell Signaling WB 1:5 000 O/N at 4 ̊C β-catenin 610153 BD Bioscience WB 1:2 000 O/N at 4 ̊C p-AKT S473 4051 Cell Signaling WB 1:1 000 O/N at 4 ̊C AKT 2920 Cell Signaling WB 1:2 000 O/N at 4 ̊C α-tubulin sc-23948 Santa Cruz Biotechnology WB 1:1 000 1h at RT mouse IgG – HRP conjugated sc-2031 Santa Cruz Biotechnology WB 1:1 000 1h at RT rabbit IgG – HRP conjugated sc-2004 Santa Cruz Biotechnology WB 1:1 000 1h at RT IHC: Immunohistochemistry; IF: Immunofluorescence; WB: Western Blot; O/N: Overnight; RT: Room Temperature ..

    Western Blot:

    Article Title: WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma
    Article Snippet: .. Protein Reference Source Technique Dilution Incubation WNT6 ab50030 Abcam IHC 1:450 O/N at 4 ̊C IF 1:1 000 O/N at 4 ̊C ab154144 WB 1:500 O/N at 4 ̊C Ki-67 550609 BD Biosciences IHC 1:200 O/N at 4 ̊C Cyclin D1 2978 Cell Signaling IHC 1:100 O/N at 4 ̊C SOX2 AB5603 EMD Millipore IHC 1:500 O/N at 4 ̊C IF 1:300 O/N at 4 ̊C BCL2 2870 Cell Signaling IHC 1:200 O/N at 4 ̊C NESTIN MAB5326 EMD Millipore IF 1:100 1h at RT rabbit IgG (H+L) – Alexa fluor® 594 A-11012 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark mouse IgG (H+L) – Alexa fluor® 488 A-11001 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark p-STAT3 Y705 9145 Cell Signaling WB 1:2 000 O/N at 4 ̊C STAT3 9132 Cell Signaling WB 1:1 000 O/N at 4 ̊C non-p-β-catenin S33/S37/T41 4270 Cell Signaling WB 1:5 000 O/N at 4 ̊C β-catenin 610153 BD Bioscience WB 1:2 000 O/N at 4 ̊C p-AKT S473 4051 Cell Signaling WB 1:1 000 O/N at 4 ̊C AKT 2920 Cell Signaling WB 1:2 000 O/N at 4 ̊C α-tubulin sc-23948 Santa Cruz Biotechnology WB 1:1 000 1h at RT mouse IgG – HRP conjugated sc-2031 Santa Cruz Biotechnology WB 1:1 000 1h at RT rabbit IgG – HRP conjugated sc-2004 Santa Cruz Biotechnology WB 1:1 000 1h at RT IHC: Immunohistochemistry; IF: Immunofluorescence; WB: Western Blot; O/N: Overnight; RT: Room Temperature ..

    Immunofluorescence:

    Article Title: WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma
    Article Snippet: .. Protein Reference Source Technique Dilution Incubation WNT6 ab50030 Abcam IHC 1:450 O/N at 4 ̊C IF 1:1 000 O/N at 4 ̊C ab154144 WB 1:500 O/N at 4 ̊C Ki-67 550609 BD Biosciences IHC 1:200 O/N at 4 ̊C Cyclin D1 2978 Cell Signaling IHC 1:100 O/N at 4 ̊C SOX2 AB5603 EMD Millipore IHC 1:500 O/N at 4 ̊C IF 1:300 O/N at 4 ̊C BCL2 2870 Cell Signaling IHC 1:200 O/N at 4 ̊C NESTIN MAB5326 EMD Millipore IF 1:100 1h at RT rabbit IgG (H+L) – Alexa fluor® 594 A-11012 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark mouse IgG (H+L) – Alexa fluor® 488 A-11001 ThermoFisher Scientific IF 1:1 000 1h at RT in the dark p-STAT3 Y705 9145 Cell Signaling WB 1:2 000 O/N at 4 ̊C STAT3 9132 Cell Signaling WB 1:1 000 O/N at 4 ̊C non-p-β-catenin S33/S37/T41 4270 Cell Signaling WB 1:5 000 O/N at 4 ̊C β-catenin 610153 BD Bioscience WB 1:2 000 O/N at 4 ̊C p-AKT S473 4051 Cell Signaling WB 1:1 000 O/N at 4 ̊C AKT 2920 Cell Signaling WB 1:2 000 O/N at 4 ̊C α-tubulin sc-23948 Santa Cruz Biotechnology WB 1:1 000 1h at RT mouse IgG – HRP conjugated sc-2031 Santa Cruz Biotechnology WB 1:1 000 1h at RT rabbit IgG – HRP conjugated sc-2004 Santa Cruz Biotechnology WB 1:1 000 1h at RT IHC: Immunohistochemistry; IF: Immunofluorescence; WB: Western Blot; O/N: Overnight; RT: Room Temperature ..



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